As the ninth-most mined metal worldwide, a large quantity of antimony (Sb) and Sb-contained compounds have been released into the environment. Sources of Sb release include abrasion from brake linings, use as flame retardants, plastic production, mining, refining and shooting activities. Though the ecotoxicity of Sb is not well known, Sb(III) compounds are generally considered to be more toxic and mobile than Sb(V), similar to that of As. Therefore, distribution, speciation, toxicity and bioavailability of Sb in various environmental compartments are considered to be primary controlling factors for human and ecosystem health. To investigate distribution and biogeochemical characteristics of Sb and other toxic metals in contaminated soils, we collected three different types of Sb-contaminated soil samples in South Korea: 1) Sb refinery, 2) Sb waste landfill site, and 3) military shooting range. Soil samples adjacent to the Sb refinery and the Sb waste landfill site generally represented much higher Sb concentration than the shooting rage. X-ray absorption fine-structure (XAFS) analysis showed that Sb was mainly in Sb(V) valence state and present as tripuhyite(FeSbO4) in sediments near refinery and landfill site. In the shooting range, multiple contamination of heavy metals from bullet was observed. Microbial population using most probable numbers (MPN) didn’t show a significant difference between sites (~106 cells/g soil). 16S rRNA gene-based metagenomic analysis were also conducted to characterize the microbial community compositions in the sites. We will discuss the possible effects of the toxic metals on the indigenous microorganisms.