Purpose: This study examines the effect of aroma inhalation therapy with lavender and rosewood essential oils for alleviating fatigue and enhancing quality of sleep in nurse shift workers. Methods: The data were collected from November 9th to 27th in 2009 at four hospitals located in Seoul. Total 60 nurses who started their night-shift works were chosen for the study and allocated to either experimental group or control group. The experimental group had had aroma inhalation therapy with lavender and rosewood for 2 weeks and was compared the level of fatigue and quality of sleep with those of the control group. Results: Significant differences were shown between the experimental group and the control group. After 2 weeks, the nurses in experimental group reported significantly lower level of fatigue and higher quality of sleep compared to the nurses in control group. Conclusion: Therefore, aroma inhalation therapy by using lavender and rosewood essential oils can be an effective intervention for relieving the level of fatigue and enhancing quality of sleepf for nurse shift workers.