The Comparison of Sequencing Method with Restriction Fragment Mass Polymorphism Method for the Detection of HBV YMDD Mutants
- Resource Type
- Article
- Authors
- An-na Jung; Hee-kyoung Jung; Hye-rim Hong; Jung-oh Park
- Source
- 임상화학검사학회 초록집 / The Korean Society for Clinical Laboratory Chemistry. Jun 04, 2005 2005(1):151
- Subject
- Language
- Korean
Background YMDD motif mutants of the hepatitis B virus (HBV) emerge in chronic hepatitis B patients after prolonged lamivudine treatment. HBV DNA breakthrough may be accompained by the emergence of YMDD mutants. We compared the performance of the sequencing method with that of RFMP method in chronic hepatitis B patients who had suffered the HBV DNA breakthrough after lamivudine treatment. Methods Samples were chosen from 20 chronic hepatitis B patients. Both sequencing and RFMP methods were used to detect YMDD variants. Results YMDD mutants were detected in 17 samples(85.0%) by both methods. Among them were two samples(10.0%) not detected mutants and in the other one sample(5.0%) were detected mutants by RFMP method. Conclusion The concordance rate between both methods was 95.0%. One sample tested by RFMP method showed an inconsistency. The major type from one sample was detected by RFMP method without a minor type However, both sequencing and RFMP methods were highly concordant except in one sample, so it is suggested that both methods are valid in detecting YMDD mutants of chronic hepatitis B patients.