Recently, the concentration of fine dustcausative substances (NOx, VOC, etc.) in the atmosphere has increased, resulting in high concentrations of tropospheric ozone (O3) and increased damage to crops. This study aimed to analyze the impact of high concentrations of ozone gas on the initial growth of rice plants and investigate the relationship between ozone damage resistance and anthocyanin biosynthesis. To achieve this, rice plants were exposed to elevated levels of ozone gas using an ozone chamber, and subsequent measurements were taken to assess changes in growth, the percentage of damaged leaves, and the anthocyanin content. The results revealed that varieties with a higher proportion of damaged leaves exhibited a relative increase in anthocyanin biosynthesis following ozone exposure. Notably, detrimental effects on growth, such as decreased biomass, were mitigated. Additionally, Anthocyanin biosynthesis genes in rice were listed by selecting homologous genes from Arabidopsis and Maize. The expression of OsF3H2, OsFLS1 and OsLDOX3 was induced during ozone treatment. This result is expected to contribute to the study of the protection mechanism of plants from ozone damage.