The world is experiencing the fourth industrial revolution, and the digital wave triggered by it has swept the whole manufacturing industry, and all countries in the world have begun to explore new formats of manufacturing enterprises under the digital background. China put forward "Made in China 2025" in 2015, and strived to become a manufacturing power by 2025. The "14th Five-Year Plan" also puts forward new requirements for Chinese enterprises to seize the opportunities of digital economy and digital technology and accelerate the digital transformation from enterprises to businesses. Supply chain management is one of the most critical links in manufacturing industry, and the key to the success of enterprise digital transformation lies in the digital transformation of supply chain. Therefore, accelerating the digital transformation of supply chain is of great significance to promote the transformation and upgrading of traditional manufacturing enterprises. Under the background of supply chain digital transformation it is of practical significance to study how to improve enterprise performance. Taking the digital transformation of enterprise supply chain as the breakthrough point, this study focuses on the theme of the influence of digital transformation of manufacturing enterprise supply chain on enterprise performance under uncertain environment, defines the concepts of core variables such as supply chain digital transformation supply chain dynamic capability and enterprise performance, and obtains the action mechanism among these variables. Based on the literature review, the research hypothesis and theoretical model of this study are put forward. Draw lessons from and refer to the mature scale of existing research, design the initial questionnaire of this study, and then optimize and improve the questionnaire through pre-investigation and expert review to form the final questionnaire. Use SPSS software to test and analyze the research hypothesis, draw corresponding conclusions and put forward countermeasures and suggestions.