Basil (Ocimum basilicum L.) is distributed worldwide and used in the food, pharmaceutical, and cosmeticindustries. Most applications are for the herb basil, recently the basil seeds have also been used commercially; however, littleis known about the nutritional and functional properties of the seeds. The present study aimed to investigate a possibleprotective effect of the methanol extract of O. basilicum seeds (MEOB), based on its phytochemical content, against kidneytoxicity induced by CCl4 in adult rats. A single dose of CCl4 was used to induce oxidative stress in rats, which wasdemonstrated by a significant rise of serum enzyme markers. MEOB was administrated for 15 consecutive days (200 mg/kgbody weight) to Wistar rats before CCl4 treatment and the effects on serum urea, creatinine, and uric acid, as well as thekidney superoxide dismutase, catalase, glutathione peroxidase, and glutathione activity and thiobarbituric acid reactivesubstances and protein carbonyl (PCO) levels were evaluated. In addition, histopathological examinations of kidneys wereperformed. In the positive control group, CCl4 induced an increase in serum biochemical parameters and triggered oxidativestress in the kidney. MEOB (200 mg/kg BW) resulted in significant reduction of CCl4-elevated levels of kidney markers, ureaand creatinine, and a significant increase of uric acid compared with the CCl4-only group. In addition, MEOB pretreatmentresulted in a significant reduction in lipid peroxidation and PCO levels in renal tissue compared with CCl4-exposed group. MEOB definitely could prevent the development of pathological changes in the kidneys. Overall, we conclude that MEOB iseffective in protecting renal function from CCl4 toxicity.