Currently, there is a lack of genetic markers capable of eff ectively detecting polymorphisms in Clematis . Therefore, wedeveloped new markers to investigate inter- and intraspecifi c diversity in Clematis . Based on the complete chloroplastgenome of Clematis ternifl ora , simple sequence repeats were explored and primer pairs were designed for all ten adequaterepeat regions (cpSSRs), which were tested in 43 individuals of 11 Clematis species. In addition, the nuclear ITS regionwas sequenced in 11 Clematis species. Seven cpSSR loci were found to be polymorphic in the genus and serve as markersthat can distinguish diff erent species and be used in diff erent genetic analyses, including cultivar identifi cation to assist thebreeding of new ornamental cultivars.