The defatted seeds of Camellia oleifera (Abel.)are used for stopping itching and pain in old days, theeffective compounds need to be investigated. Sasanquasaponinas a rich fraction was extracted with 70 % ethanol,purified by AB-8 macro-reticular resin, crystallized in 80 %ethanol, and further hydrolyzed by 4 % hydroxyl potassiumor 2 M hydrochloride. Anti-inflammatory activities of theextracts were measured by carrageenan-induced paw edemain rats and croton oil induced ear inflammation in mice; theanalgesic activities were analyzed by hot plate test, aceticacid induced writhing in mice; the levels of pain mediatorsof IL-1b, TNF-a and PGE2 were determined; the antioxidativeactivities in vivo were evaluated by MDA, SOD andGSH-Px in serum of rats. The extracts showed significant(p\0.01) anti-inflammatory and analgesic activities,remarkably (p\0.01) inhibited production of pro-inflammatorycytokines and PGE2, decreased MDA and increasedSOD and GSH-Px in serum. Inhibition of IL-1b, TNF-a andPGE2 may contribute to their anti-inflammatory and analgesiceffects; elimination of free radicals is also involved. The sapogenin and acid hydrolyzed product have betteranti-inflammatory, analgesic effects, and stronger antioxidativeactivity than sasanquasaponin and alkaline hydrolyzedproduct, and they are better candidate medicines forinflammation and pain.