Modern long-span floor system typically possesses low damping and low natural frequency, presenting a potential vibration sensitivity problem induced by human activities. Field test and numerical analysis methods are available to study this kind of problems, but would be inconvenient for design engineers. This paper proposes a simplified method to determine the acceleration amplitudes of long-span floor system subjected to walking or running load, which can be carried out manually. To theoretically analyze the acceleration response, the floor system is simplified as an anisotropic rectangular plate and the mode decomposition method is used. To facilitate the calculation of acceleration amplitude aP, a coefficient αwmn or αRmn is introduced, with the former depending on the geometry and support condition of floor system and the latter on the contact duration tR and natural frequency. The proposed simplified method is easy for practical use and gives safe structural designs.