Two-dimensional layered double hydroxides (LDH) as a membrane material has tremendous potential inthe water treatmentfield. However, the stability and permeance of the LDH membrane should be furtherimproved for the industrial application. In this study, the LDH separation layer was in situ grown on thetubular Al2O3 substrate. The obtained LDH composite membrane was then calcined to reduce the surfacefree energy and remove some interlayer anions. Therefore, the calcined LDH composite membraneexhibited enhanced stability and permeance for removing dye molecules from water. The permeance ofthe calcined LDH membrane reached 367 L m 2 h 1MPa 1 and the rejection for Eriochrome black T wasstill above 99%. Moreover, the membrane can maintain the separation performance more than 110 h inthe nanofiltration process. The morphologies and structures of the LDH membranes before and aftercalcination were also investigated. The results indicated that this facile method is effective to improve theseparation performance of the LDH membrane.