Tomato cultivation at lower or higher temperatures than the optimum negatively afects plant growth and development. Large diferences in abiotic stress tolerance have been found between Solanum lycopersicum and wild tomato species. Ouraim was to compare temperature stress tolerance in cultivated and wild tomato genotypes to identify cold- and heat-toleranttomatoes for further utilization in tomato breeding. The maintained net photosynthetic rate (PN) and chlorophyll fuorescencewas related to the tolerance of tomatoes at temperature stress. The PN and chlorophyll fuorescence of one cultivated tomato(Ly from S. lycopersicum) and six wild tomatoes genotypes (Ha from Solanum habrochaites, Pe from Solanum pennellii,Pi1 and Pi2 from Solanum pimpinellifolium, Pr1 and Pr2 from Solanum peruvianum) grown at low (12 °C) and high (33 °C)temperatures were compared. The PN of four tomato genotypes during temperature stress were lower than the control, butnot in Pe, Pr1, and Pr2. The maximum quantum efciency of photosystem II (Fv/Fm) of the cultivated tomatoes was lowerat both 12 and 33 °C than the control using Handy PEA, whereas Fv/Fm using MINI-PAM was lower only at 12 °C. Thechlorophyll fuorescence OJIP transient (OJIP curve) revealed diferences between temperature stress responses and tomatogenotype. With the exception of Pr2, the Fv/Fm in wild tomatoes was unafected by temperature stress; however, they stillmaintained clear genotype diferences for other physiological traits such as PN, quantum yield of PSII (Fq′/Fm′), electrontransport rate, non-photochemical quenching, and the fraction of open PSII centers (qL). These results indicated that the wildtomato varieties Pe and Pr1 had the highest temperature stress tolerance, while the cultivated species was the more sensitiveto temperature stress in comparison. In general, the wild tomato genotypes were more tolerant to both cold and heat stressthan the cultivated tomato, suggesting that these wild species could be used to uncover underlying mechanisms of temperaturestress tolerance and will be promising sources of genetic variability for temperature stress tolerance in breeding programs.