The present study was planned to analyze the nutritional quality, microbial counts and fermentative acids in Italian ryegrass(IRG) 80% and alfalfa 20% (IRG-HV) mediated silage inoculated with lactic acid bacteria (LAB) as a probiotic strain for 3months. Crude protein (CP), acid detergent fiber (ADF), and neutral detergent fiber (NDF), total digestible nutrient (TDN) and Invitrodry matter digestibility (IVDMD), lactic acid bacteria (LAB), yeast and fungi counts and fermentation metabolites such aslactic acid, acetic acid and butyric acids were analyzed. The result shows that the nutritional quality and metabolite profiles ofsilage were significantly improved with LAB. For microbial counts, LAB showed dominant followed by yeast as compared withcontrol silage. The pH of the silage also reduced significantly when silage inoculated with LAB. The result confirmed that silagepreparation using different crops with L. plantarum inoculation is most beneficial for the farmers.