Tea is one of the most consumed beverages worldwide and the relatively high levelspolyphenols is benefit for health. In this study, we developed an efficient system for proliferationof callus from ‘Anji Baicha’, a cultivar of tea (Camellia sinensis ). Callus tissue was initiallyinduced by culturing leaf explants on medium containing different plant growth regulators. Forcallus induction, thidiazuron (TDZ) was more effective than 2,4-dichlorophenoxyacetic acid(2,4-D), α-naphthalene acetic acid (NAA), and N6-benzyladenine (BA). The frequency of callusinduction from leaf explants reached 90.21% on 1.0 mg·L-1 TDZ and the developed calluswas reddish and friable. We also tested the effect of different concentrations of NAA, 2,4-D,indole 3-acetic acid (IAA), BA, and TDZ, alone and in combinations, on callus proliferation. Medium supplemented with TDZ in combination with IAA was suitable for callus proliferationand accumulation of tea polyphenols. The growth index value and tea polyphenol content ofcallus cultured on MS medium containing 0.5 mg·L-1 TDZ and 1.0 mg·L-1 IAA was maximally1,351% and 23.24%, respectively, and the relative abundance of epicatechin was as high as17.449%. We also measured the antioxidant activity of all samples and the callus with thehighest tea polyphenol content also exhibited high potential radical scavenging activity.