Bacterial infection is one of the major threats to human health worldwide, posing a great challenge to thedevelopment of medical protective materials with efficient filtration and long-lasting antimicrobial properties. In this study, electrospinning technology and non-woven fabrics were combined to construct amicro/nano-structured photodynamic rechargeable and storable antibacterial filtration composite membrane. The filtration efficiency of the composite membrane is as high as 99.99% for 0.5–5 lm particles,and the resistance pressure drop is 85 Pa. In addition, the release of OH and H2O2 from the compositefilm reached 5326.16 lg/g and 711.93 lg/g, respectively. After 7 cycles of quenching, the charging capacityretains more than 70 % of the original. It showed good antimicrobial properties even when storedunder dark conditions for one month. The bactericidal efficiency of the composite membrane againstE. coli and S. aureus exceeded 99% under both dark and light conditions. The development of this simpleand clean micro/nanofiber membrane provides a new idea for exploring photoactive antimicrobial andfiltration materials for medical protection.