This study was conducted to identify thequantitative trait loci (QTL) associated with grain yield andits related traits by analysis of 122 doubled haploid populationderived from a cross between Korean wheat cultivarscarried different yield potential. DH population was evaluatedin replicated field trials in 2011/2012 and 2012/2013at upland and paddy conditions in Iksan, Korea. Grain yieldand its related traits, including days to heading and maturity,culm and spike length, spike number per m2, kernelnumber per spike, and thousand kernel weight, wereinvestigated. A genetic map was constructed with 140microsatellite marker loci and a total of nine QTLs wereidentified on eight chromosomes for grain yield and itsrelated traits. The three QTLs, Xgpw2206, Xgwm247, andXwmc471, were detected on chromosomes 2A, 3A, and 3B,and explained 10.1–22.8 % of phenotypic variation forgrain yield. Among these QTLs, Xgpw2206 and Xgwm247explained 10.7–37.9 % of phenotypic variation for days toheading and maturity, spike length, spike number per m2,and kernel number per spike. Other six QTLs on chromosomes2D, 4A, 5A, 6A, and 7A accounted for 11.4–39.9 %of phenotypic variation for culm and spike length, spikenumber per m2, and thousand kernel weight. Those threeQTLs for grain yield were applied to validate relationshipbetween genotypes of QTL and 25 Korean wheat cultivars. Korean wheat cultivars carried hha genotypes based on theorder of those three QTLs, showed higher GY (664.4 kg/10a) than genotype bha (494.2 kg/10a). Those three QTLscould be used in marker-assisted selection to improve grainyield and its related traits in Korean wheat breedingprograms.