Tobacco hydroxyproline-rich glycopeptide systemin prec~ursor A (TobpreproHypSys-A), fmlrn which TobHypSys I and I! are released, plays a crucial role in defense responses. Here, we i~vestigated the expression o~ TobpreproHypSys-A and the activity of defense proteins in tobacco organs during wounding. Expression was induced mo~,'.~ rapidly in upper, non-wounded leave~ than in lower, wounded leaves. At 24 h after mechanical wounding, expression wa~ I[ow in the roots, but increased in the stems and flowers, although to a lesser extent than i~ the leaves. At 3 or 10 d after i~sect-woundir~g, expression did not differ among organs, suggesting that TobpreproHypSys~A could be induced globally and ce~tinuously throughout sucl~ stress. During that period, the activiity of two defense proteins -- PPO and PI -- was consistent with the expression of TobpreproHyp- Sys-A in various organs. This indicates that those proteins also could be regulated by TobHypSys, both globally and continuously.