Purpose: Multiple pathways are involved in inducing liver fibrosis, which can damage the integrity of liver. Among them, miR-125b has been found to exert an activating action on hepatic stellate cells. Endoplasmic reticulum stress and autophagy lead toliver disorders. Here, we evaluated the therapeutic influence of miR-125b on the endoplasmic reticulum function in injured liverssubmitted to bile duct ligation. Materials and Methods: For inducing injury, bile duct ligation was done on miR-125b transgenic rats (miR-125b-Tg) in wild typerats. The rat T-6 cells received transfection of miR-125b mimic and Tunicamycin. Protein expressions were observed by westernblot analysis. Results: Compared to wild type rats, liver-injured rats showed significant impairment of liver function as assessed by the total bilirubinlevels. The miR-125b-Tg rats showed decrease in activity of aspartate transaminase and alanine transaminase. Liver tissuesof miR-125b-Tg rats showed weaker fibrotic matrix formation. Upregulation of miR-125b decreased the bile duct ligation-mediatedhepatic disturbances for the expressions of endoplasmic reticulum kinase, inositol-requiring kinase 1alpha, sXBP1, CHOP, LC3,p62, ULK, and caspase-3/-8/-9. T-6 cells transfected with miR-125b mimic and treated with Tunicamycin caused decrease in levelsof cleaved caspase-3, sXBP1, CHOP, and LC3. The miR-125b signaling showed protective effect on the liver tissues subjected to injuryand fibrosis histopathology. Conclusion: This study demonstrates a novel insight into the miR125b-mediated stabilization of endoplasmic reticulum integrity,which slows the progression of injury-induced hepatic deterioration.