Lactiplantibacillus plantarum CKDB008 was isolated from a Korean fermented food, kimchi. In order to investigate its potential as a probiotic in the food industry, the genome sequence of L. plantarum CKDB008 was analyzed to confirm its probiotic properties and safety at the genome level. The CKDB008 strain has one circular chromosome of 3.2 Mbp, with 44.6% guanine-cytosine content. A comparative genomic analysis confirmed that its genome was similar to those of 19 L. plantarum strains used as probiotics. Pan-genome analysis of 20 L. plantarum strains identified 6,236 genes, including 2,006 core, 255 soft core, 1,485 shells, and 2,490 cloud genes. These results reveal the ‘open’ nature of L. plantarum pan-genome. Among the predicted 3,129 protein coding genes, the genome contains genes associated with important characteristics of probiotics such as protease system, amino acid biosynthesis, major carbohydrate-active enzymes, stress resistance, and cell adhesion. In addition, genes encoding bacteriocins, immunoregulatory, and gamma-aminobutyric acid biosynthesis were also identified, but safety-related genes related to antibiotic resistance and virulence factors were not. The results obtained strongly suggest that CKDB008 strain is genetically safe and has the desired properties required for a probiotic as well as support that L. plantarum CKDB008 strain has a potential as a probiotic that can be used for the industrial purpose.