In this study, to impart silkfibers with excellent coloration, UV-protection, antimicrobial activity, as wellas creatinine adsorption from artificial blood, silkfibers were designed via facile fabrication approach. These diverse properties were introduced by in-situ incorporation of nanoparticles based on either silver(AgNPs) or gold (AuNPs) as well as bimetallic (Ag-AuNPs) onto silkfibers using an eco-friendly, effectiveone-pot process. The proteins present in the silkfibers acted as a redox-active bio-template to reduce theAg(I) and Au(III) ions to their zero-valent state with simultaneous deposition of the generatednanoparticles onto the silkfiber surfaces. The obtained results clearly indicated that both AgNPs andAuNPs colored the silkfibers with brown and red color, respectively, thereby affirming the deposition ofthese metallic NPs onto and inside the surface of silkfibers as S/Ag-AuNPs. Significantly, the preparedcolored silkfibers exhibited excellent antimicrobial and cytotoxicity activities against differentpathogenic microbes as well as very good UV protection properties. Moreover, the results attestedthat the as prepared S/AgNPs and S/AuNPsfibers have good adsorption potential with maximaladsorption capacity of creatinine in artificial blood was found to be 94.2 mg/g for S/AuNPs sample, whichis 1.55 times greater than that of silkfibers.