OBJECTIVE: To create and evaluate an umbilical catheterization task trainer using a human umbilical cord. DESIGN: We obtained human umbilical cords and attempted to process and preserve them. We used the following four solutions for preservation in a freezer: Ringer’s acetate solution with and without heparin and saline solution with and without heparin. Two experienced neonatologists discussed which of these four types of defrosted cords is the best from the viewpoints of appearance, elasticity, and patency. Less-experienced pediatricians and neonatologists participated in the training for umbilical catheterization with the best cord. Thereafter, the learners evaluated the training in terms of self-confidence, satisfaction, clinical application, and self-efficacy. RESULTS: The cords soaked in Ringer’s solution with heparin were judged as the best. With use of them, all the learners were able to insert catheters into both the umbilical artery and vein. The average scores of the learners’ evaluations were all greater than four of five points. CONCLUSION: Human cords prepared by our method functioned as highly effective task trainers for umbilical catheterization and received high praise from learners.