This report describes the applications of various cationic polymer beads for chromatographic separation of DNA from protein-containing solutions. The beads were prepared by introduction of polycation onto chloromethyloxirane-activated cellulose beads or copolymerization of N,N-dimethylaminopropylacrylamide(DMAPAA) and DVB. We will first discuss chromatographic properties of various DNA-specific polymer adsorbents, and then describe effects of pore size of adsorbent, its amino-group content, its surface pKa value, and condition of buffer on separation of DNA from a protein solution.