To evaluate the effects of voice therapy for 35 patients with vocal fold nodules performed in our clinic, we measured voice handicap index (VHI) and maximum phonation time (MPT) before and after the therapy and analyzed the results. GRBAS scale was also used, and for acoustic aspects, jitter, shimmer, noise-to-harmonic ratio (NHR), and fundamental frequency (F0) were evaluated. We observed significant improvements in VHI, MPT, and shimmer, and also a significant increase in F0 after voice therapy. No significant changes in jitter or NHR were observed after voice therapy when comparing all patients as one group, but patients whose jitter or NHR measurement before the therapy was over the cutoff values showed a significant improvement.