Spectrophotometric Determination of Tetramine in Carnivorous Gastropods with Tetrabromophenolphthalein Ethyl Ester
- Resource Type
- Journal Article
- Authors
- Etsuo MORI; Kouki TANAKA; Mitsuji SAITOU; Naoko MORIWAKI; Reiko FUJII; Tokihiko OGAWA
- Source
- Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi). 1992, 33(3):237
- Subject
- gastropods
ion association reagents
salivary gland
tetrabromophenolphthalein ethyl ester
tetramine
イオン会合性試薬
テトラブロモフェノールフタレインエチルエステル
テトラミン
唾液腺
巻貝
- Language
- English
- ISSN
- 0015-6426
1882-1006
An analytical method of tetramine (tetramethylammonium, TM) in the salivary gland of gastropods with tetrabromophenolphthalein ethyl ester (TBPE) as an ion-pairing counter-ion was developed.TM was extracted into 1, 2-dichloroethane with TBPE under strongly alkaline conditions. Other quaternary ammonium species and amines tested did not interfere with the determination of TM. The ion-associate showed an absorption maximum at 610nm. Recovery of TM-Cl from the salivary gland was 100.2±2.3% and the determination limit was 0.20mg/g.Contents of TM-Cl in the salivary gland of gastropods were in the range of 2.39-6.08mg/g