Identification of Oil-Soluble Coal Tar Dyes in Cosmetics Using Reversed-Phase TLC/Scanning Densitometry
- Resource Type
- Journal Article
- Authors
- Eiichi Mikami; Hiroshi Matsumoto; Tsutomu Ohno
- Source
- Journal of Health Science. 2003, 49(5):401
- Subject
- cosmetic
oil-soluble coal tar dye
reversed-phase TLC
scanning densitometry
- Language
- English
- ISSN
- 1344-9702
1347-5207
A simple and rapid analytical method of oil-soluble coal tar dyes in cosmetics was established using reversed-phase TLC/scanning densitometry. Eleven kinds of oil-soluble coal tar dyes were able to be separated completely on reversed-phase TLC plates by the complementary use of 2 solvent systems. The solvent systems were A; n-hexane/2-butanone solution (5 : 1, v/v), solvent system B; acetonitrile/methanol solution (5 : 1, v/v). Then we measured visible absorption spectra of spots developed on the reversed-phase TLC plates by scanning densitometer to identify these coal tar dyes. The proposed method was successfully applied to the identification of oil-soluble coal tar dyes in commercial cosmetics.