Suppressive Effects of Self-Associated Triblock Copolymers on Quorum Sensing-Mediated Phenazine Antibiotic Production
- Resource Type
- Journal Article
- Authors
- Chigusa Okano; Norihiro Kato
- Source
- Transactions of the Materials Research Society of Japan. 2016, 41(2):163
- Subject
- N-acylhomoserine lactone
cell-cell communication
quorum sensing
self-associated micelle
triblock copolymer
- Language
- English
- ISSN
- 1382-3469
2188-1650
Effective suppression of a bacterial quorum sensing (QS) could be achieved by adding A-B-A tri-block copolymer, poloxamer 84 or 407 into a culture broth, resulting in decrease of the QS-mediated phenazine production in Pseudomonas chlororaphis subsp. aurantiaca StFRB508. Dynamic light scattering measurements suggested to form self-associated micelles of respective poloxamer, because a single peak in the size distribution was observed under the same concentration (1 wt%) and temperature (30℃) for the QS suppressive test. The phenazine production in StFRB508 strain was known to be induced by N-hexanoylhomoserine lactone (C6HSL) and 3-hydroxy-hexanoylhomoserine lactone (3OH-C6HSL) as QS signaling molecules which can activate an expression of phenazine-related gene after sufficient increase of cell density. The micelles of poloxamer 84 and 407 could suppress the induced phenazine amounts to 55 and 60%, respectively. An N-acylhomoserine lactone (AHL) bioassay with Chromobacterium violaceum CV026 suggested effective trap of AHL derivatives to poloxamer micelles because the AHL concentration in the culture broth was obviously reduced in the presence of poloxamers.