Karyotypic analysis of Gossypium herbaceum and Gossypium arboreum has been made taking the parameters like number of chromosomes having secondary constriction and satellite, position of centromere i. e. number of median, submedian and subterminal chromosomes, F%, TF%, relative length of individual chromosomes and relative length of shortest chromosome. It is concluded that at species level G. herbaceum is primitive to G. arboreum and at racial level persicum is primitive to wightianum in G. herbaceum, and indicum is primitive to bengalense in G. arboreum.