Evidence from Nondenaturing Filter Elution That Induction of Double-Strand Breaks in the DNA of Chinese Hamster V79 Cells by γ Radiation Is Proportional to the Square of Dose
- Resource Type
- research-article
- Authors
- Blazek, Ed Robert; Peak, Jennifer G.; Peak, Meyrick J.
- Source
- Radiation Research, 1989 Sep 01. 119(3), 466-477.
- Subject
- Elution
Dose response relationship
Radiation dose response relationship
DNA
Radiation dosage
DNA damage
Cell lines
Calibration
DNA repair
Radiation damage
- Language
- English
- ISSN
- 00337587
19385404
We have used nondenaturing filter elution performed at both pH 7.2 and pH 9.6 to measure the induction of double-strand breaks (DSBs) in the DNA of Chinese hamster V79 cells by 60 Co γ-radiation doses between 10 and 120 Gy. The absolute DSB yields as measured by this assay were determined by using our recent calibration of the assay based upon disintegrations of 125 I incorporated into the DNA. An analysis of the dose-response relationship for the induction of DSBs by 60 Co γ rays showed that the number of DSBs induced per dalton of DNA was proportional to the square of the applied dose throughout the dose range used. The contribution made by the dose to the first power was small at pH 9.6 and negligible at pH 7.2. These results suggest that DSB induction in cells by γ rays may be entirely a two-hit event.