目的: 本研究使用人角质形成细胞和黑色素瘤在体外验证水解小雨燕窝提取物(HSNE)的皮肤美白和保湿效 果。方法: 为了确认HSNE的抗氧化作用,测量了DPPH 自由基清除活性。为了找出HSNE的美白效果,测量了 与黑色素生成相关的基因、酪氨酸酶(TYR )、酪氨酸酶相关蛋白(TRP )1 、2 和MITF 的mRNA表达。并测 量了HSNE处理后的黑色素含量,以确认抗黑色素生成作用。使用逆转录聚合酶链反应 (RT-PCR),确定了与 保湿相关的基因,例如水通道蛋白 (AQP )3 、透明质酸合成酶(HAS )1 、2 和3 。测试结果用student's t -test分 析并表示为平均值±标准差。结果: HSNE的DPPH自由基清除作用以浓度依赖性方式增加。此外,通过降低 TYR 、TRP1 、TRP2 和MITF 的表达,抑制黑色素生物合成,证实具有美白作用。保湿相关基因(HAS1 、2 、3 和 AQP3 )的表达以浓度依赖性方式增加,证明其保湿效果。结论: 经证实水解小雨燕窝提取物具有美白保湿作 用,可作为功能性化妆品原料。
Purpose: This research verified the skin whitening and moisturizing effects of hydrolyzed swiftlet nest extracts (HSNE) in vitro using human keratinocytes and melanoma. Methods: To confirm the antioxidant effect of HSNE, DPPH radicalscavenging activity was measured. To find out the whitening effect of HSNE, the genes related to melanogenesis, mRNA expression of tyrosinase (TYR ), tyrosinase related protein (TRP ) 1 , 2 and microphthalmia associated transcription factor (MITF ) were measured. We also measured the melanin contents after treatment of HSNE to confirm the anti-melanogenesis effect. Using reverse transcription polymerase chain reaction (RT-PCR), the genes related to moisturizing such as aquaporin (AQP ) 3, hyaluronan synthase (HAS ) 1 , 2 , and 3 were determined. The results of the tests were analyzed with student’s t-test and expressed as mean±standard deviation. Results: DPPH radical scavenging effects of HSNE increased in a concentration dependent manner. The expression of melanogenesis-related genes were inhibited by the treatment of HSNE in a concentration-dependent manner (MITF , TYR , TRP1 , and 2 ). Melanin contents also decreased with the treatment of HSNE. The expression of moisturizing-related genes (HAS1 , 2, 3, and AQP3 ) increased in a concentration-dependent manner. Conclusion: It is confirmed that the hydrolyzed swiftlet nest extracts have skin whitening and moisturizing effects and can be used as a functional cosmetic raw material.