目的: 目前,与衰老相关的肌肉减少症被认为是一个严重的问题。然而,很少有研究涉及使用 双斑蟋蟀治疗或 预防肌肉减少症。因此,本研究旨在确定双斑蟋蟀是否对肌肉减少症有改善作用,并研究其作为食物对老年人 或患有这种疾病的患者的肌肉减少症的适用性。方法:分析了水分、粗灰分、粗蛋白质、粗脂肪、碳水化合物 和矿物质的含量。分别使用自动氨基酸分析仪和气相色谱法分析氨基酸和脂肪酸组成。使用 3-(4,5-二甲基噻唑 -2-基)-2,5-二苯基溴化四唑 (MTT) 测定法测量细胞活力。在用双斑蟋蟀提取物处理后,使用荧光素酶报告基因 测定在 C2C12 细胞中研究肌生长抑制素表达。结果:双斑蟋蟀粉 (GBP)、双斑蟋蟀蛋白提取物 (PEGB)、双斑 蟋蟀卵(GBE)和双斑蟋蟀卵蛋白提取物(EPGB)富含必需氨基酸,尤其是支链氨基酸和不饱和脂肪酸,碳水化合物 含量低。此外,双斑蟋蟀似乎通过抑制C2C12细胞中肌肉生长抑制素的mRNA 表达来改善肌肉减少症。结论: 双斑蟋蟀将对食品材料的开发或预防老年人或患有这种疾病的患者的肌肉减少症产生积极影响。
Purpose: Currently, aging-related sarcopenia is considered a serious problem. However, few studies have addressed the treatment or prevention of sarcopenia using Gryllus bimaculatus . Therefore the present study was carried out to determine whether Gryllus bimaculatus has an ameliorative effect on sarcopenia and to study its applicability as a food for sarcopenia in the elderly or in patients with this condition. Methods: The contents of moisture, crude ash, crude protein, crude fat, carbohydrate and minerals were analyzed. The amino acid and fatty acid compositions were analyzed using an automatic amino acid analyzer and gas chromatography, respectively. Cell viability was measured using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Myostatin expression was investigated in C2C12 cells using a luciferase reporter assay after treatment with a Gryllus bimaculatus extract. Results: The Gryllus bimaculatus powder (GBP), protein extract of Gryllus bimaculatus (PEGB), Gryllus bimaculatus egg (GBE), and egg protein extract of Gryllus bimaculatus (EPGB) were high in essential amino acids, especially branched chain amino acids, and unsaturated fatty acids, and low in carbohydrates. Moreover, Gryllus bimaculatus appeared to have ameliorative effects on sarcopenia by suppressing the mRNA expression of myostatin in C2C12 cells. Conclusion: It is expected that Gryllus bimaculatus will be positive for the development of food materials or to prevent sarcopenia in the elderly or in patients with this condition.