目的: 为了确认混合植物根提取物和发酵混合植物根提取物的生物活性,通过体外实验证实了抗衰老和抗炎作 用。方法: 通过对DPPH自由基清除活性的评价,筛选出具有优异抗氧化活性的植物根提取物,并利用所选择的 植物根混合提取物(MC)和酿酒酵母制备了混合植物根提取物(MF)的发酵产物。确认MC和MF对人皮肤成纤维细 胞COL1A1 和HAS2 mRNA表达的影响,并通过细胞增殖实验评价了细胞迁移能力。为了证实 MC和MF的抗炎作 用,通过RT-PCR证实了 COX2 、TNF-α 、IL6 在人角质形成细胞中的表达。实验结果用平均值和标准差表示,结 果采用t检验分析。结果: 通过对DPPH自由基清除活性的评价,选择了15种根提取物中的5种。 MC和MF以浓度 依赖性方式增加COL1A1 和HAS2 的表达,并表现出细胞增殖作用。此外,MC和MF以剂量依赖性方式抑制炎症 相关基因TNF-α 、IL6 、COX2 的表达。在所有实验中,与MC相比,MF显示出更好的效果。结论: 蒲公英根茎/根 提取物、牛蒡根提取物、知母根提取物、野葛根提取物和莲根提取物的混合发酵产物具有抗衰老和抗炎作用, 可作为功能性化妆品原料应用。
Purpose: To verify the biological effects including anti-aging and anti-inflammatory effects, of fermented plant root extract mixtures that were measured in vitro . Method: To select the most effective plant root extract, the effects of DPPH free radical scavenging were measured. The mixture (MC) of selected plant roots was fermented with Saccharomyces cerevisiae (S. cerevisiae ) (MF). Reverse transcription (RT)-PCR was performed on human dermal fibroblast to measure the effects of MC and MF on the mRNA expression of COL1A1 and hyaluronic acid synthase 2 (HAS2 ) and on the anti-inflammatory-related gene expressions such as cyclooxygenase-2 (COX-2 ), interleukin-6 (IL-6 ), and tumor-necrosis factor (TNF )-α . Additionally, we also tested the cell renewal and proliferation for skin cell migration. Student's t-test was performed for statistical analysis, and results were expressed as mean ± standard deviation. Results: Among 15 root extracts, those of Taraxacum officinale (T. officinale ) rhizome, Arctium lappa (A. lappa ), Anemarrhena asphodeloides (A. asphodeloides ), Pueraria lobata (P. lobata ), and Nelumbo nucifera (N. nucifera ) were selected based on the effect of DPPH free radical scavenging. The mRNA expression levels of COL1A1 and HAS2 were increased by MC and MF in a concentration-dependent manner. MC and MF showed cell renewal and proliferation. Inflammation-related genes were inhibited by MC or MF in a concentrationdependent manner (TNF-α , IL-6 , and COX-2 ). MF showed significantly better efficacy than MC. Conclusion: Mixed root extracts of T. officinale rhizome/root , A. lappa , A. asphodeloides , P. lobata , and N. nucifera fermented with S. cerevisiae enable cell renewal, have anti-aging and anti-inflammatory effects, and can be used as an active cosmetic raw materials.