표고수확후배지 퇴비 물 추출물에서 Bacillus subtilis BSM 320의 고밀도 배양 및 표고 푸른곰팡이병의 생물학적 방제
High density culture of Bacillus subtilisBSM320 in aqueous extract of composted spent mushroom substrate of Lentinula edodes and biological control of green mold disease
- Resource Type
- Article
Text
- Authors
- 김자윤; 박세현; 박성준; 황보주형; 강희완; Ja-Yoon Kim; Se-Hyun Park; Seong-Joon Park; Ju-Hyeong Hwang Bo; Hee-Wan Kang
- Source
- 한국버섯학회지, 09/30/2023, Vol. 21, Issue 3, p. 140-144
- Subject
- Bacillus subtilis BSM320
Biological control
Green mold
- Language
- Korean
- ISSN
- 1738-0294
The objective of this study was to achieve biological control of green mold disease in Pyogo mushrooms using antagonistic microorganisms. Bacillus subtilis BSM320 cells inhibited mycelial growth by 48–60% against three Trichodermaisolates including T. hazianumisolated from the substrates of Lentinula edodes, showing their antifungal activity.The bacteria were cultured to a high density of 4.2 x 109±113.7 cfu/mlin aqueous extract of composted spent mushroom substrates of L. edodes containing 1% glucose and showed a higher growth rate than that observed when using the commercial medium, Luria-Bertani broth. The bacterial culture showed a 75% protective effect without damaging the mushroom fruiting bodies. These results suggest that B. subtilis BSM320culture is suitable for biological control of green mold disease during mushroom cultivation.