The previously reported acoustofluidic tweezers(AFTs) array chip has been demonstrated for rapid enrichment of micro/nanoparticles. However, the chip operates discretely, resulting in limited throughput.This article presents a microfluidic chip fabricated using six layers of bonded polydimethylsiloxane(PDMS). The chip incorporates pneumatic microvalves that enable the switching of two outlets by controlling the pneumatic valves. Furthermore, a comprehensive control system was devised encompassing components such as microscope, microcontroller, computer, signal generator, power amplifier, syringe pump, and air pump. This system facilitates the automation and intelligence of high-throughput submicron/nanoparticle screening.With the aid of the smart microfluidic system, the processing speed of the sample by AFTs can attain the rate of approximately 1-2 mL/h.