The non-contact strain sensor based on the single-walled carbon nanotube (SWCNT) photoluminescence effect has a great potential that attracted extensive research from both domestic and international scholars. In order to research the non-contact strain sensor, the current paper proposed a fluorescence excitation and acquisition system platform for SWCNT strain sensors, and designed the structures of its optical components. The design, installation, and debugging of this system are conducted. Results show that the fluorescence excitation and acquisition system established in this paper is a suitable platform for SWCNT optical full field strain measurement. Based on this system, the fluorescence excitation and acquisition of the SWCNT film are simulated and analyzed by using OpticStudio software. Simulation results show that the fluorescence intensity is in partial positively correlated with the excitation light intensity, and the light spot diameter could be controlled within 0.1mm. The light spot scanning resolution of the SWNT film achieved the expected effect.