In order to test the effect of online physical training for college students under the epidemic. We selected the college students from Guangzhou X College as the experimental samples, and conducted a study on the distinction between the experimental group and the control group. In the experimental group, the basic physical condition was learned through Functional Movement Screening (FMS). Then carried out cause analysis and functional adjustment exercises for low-scoring items. Based on the weakness of upper limbs and core strength discovered by the experimental group during the test, we conducted targeted online physical training for 8 weeks. A comparative analysis based on the physical test before and after training and the FMS score of the experimental group. After training intervention, it has a significant impact on Shoulder Mobility, Active Straight Leg Raise, Rotary Stability and total score in FMS, but it has a counter-effect on the Hurdle Step, and other test actions have different gender validity. Through the physical test, the data of the experimental group has been significantly improved (P