Relationship between Escherichia coli Sterilization in Ice and Electric Field Application
- Resource Type
- Conference
- Authors
- Watanabe, Masato; Hikosaka, Yukiko; Murakami, Yuichi; Muramoto, Yuji
- Source
- 2022 IEEE Conference on Electrical Insulation and Dielectric Phenomena (CEIDP) Electrical Insulation and Dielectric Phenomena (CEIDP), 2022 IEEE Conference on. :414-417 Oct, 2022
- Subject
- Engineered Materials, Dielectrics and Plasmas
Insulation
Microorganisms
Electric breakdown
Cells (biology)
Maintenance engineering
Electrophoresis
Ice
- Language
- ISSN
- 2576-2397
This study explored the relationship between sterilization of Escherichia coli in ice and electric field application. The experimental sample was a 0.01 [mol/L] NaCl solution containing E. coli. This sample was frozen at -20[°C], following which the sample was subjected to a DC electric field of 1– 150[V/mm] at -20[°C]. Consequently, the survival ratio of E. coli after only freezing and application of electric field was lower than that of no-treatment. Notably, E. coli cells were damaged by freezing and electric field application. Electrophoresis indicated that more nucleic acids were detected after DC electric field application and after only freezing.