Oxygen Detection of Single Cells with Guided Movements
- Resource Type
- Conference
- Authors
- Wang, Muting; Pang, Stella W.
- Source
- 2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers) Solid-State Sensors, Actuators and Microsystems (Transducers), 2023 22nd International Conference on. :963-966 Jun, 2023
- Subject
- Bioengineering
Components, Circuits, Devices and Systems
Fields, Waves and Electromagnetics
Photonics and Electrooptics
Robotics and Control Systems
Oxygen
Biomedical optical imaging
Transducers
Tissue engineering
Fluorescence
Optical imaging
Time measurement
Oxygen Detection
Optical Oxygen Sensor
Guided Movements
MC3T3-E1 Cell
- Language
- ISSN
- 2167-0021
Measurement of oxygen (O 2 ) consumption is a critical parameter for understanding cell behaviors and viability in many biological systems. In this study, an O 2 detection platform that comprises a luminescent dye-based optical O 2 sensor and guiding structures was presented. This platform was employed to investigate the relationship between the O 2 consumption of single cells and cell movements. Real-time observation of changes in O 2 consumption was achieved as single cells were guided by patterned structures using the proposed O 2 detection platform. The results demonstrate that changes in O 2 consumption were associated with the area of mitochondria within the cell. Specifically, O 2 consumption increased as the area occupied by mitochondria increased, as evidenced by an increase in the fluorescence intensity of the optical O 2 sensor. These findings lay the foundation for studying cell activities and viability in tissue engineering.