Towards artificial cell array system: Encapsulation and hydration technologies integrated in liposome array
- Resource Type
- Conference
- Authors
- Osaki, Toshihisa; Kamiya, Koki; Kawano, Ryuji; Sasaki, Hirotaka; Takeuchi, Shoji
- Source
- 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems (MEMS) Micro Electro Mechanical Systems (MEMS), 2012 IEEE 25th International Conference on. :333-336 Jan, 2012
- Subject
- Fields, Waves and Electromagnetics
Computing and Processing
Components, Circuits, Devices and Systems
Communication, Networking and Broadcast Technologies
Lipidomics
Arrays
Substrates
Electrostatic discharges
Nanobioscience
Encapsulation
Films
- Language
- ISSN
- 1084-6999
This work presents two important technologies integrated in our uniform-size liposome array platform (Fig. 1a) to realize an artificial cell array system. One is the effective encapsulation of small objects in the arrayed liposomes (Fig. 1b top), making use of the electrospray deposition technique also used for the lipid patterning. The target nanobeads were selectively patterned on the lipid and encapsulated in the liposomes. Another technology is the liposome formation under biological conditions (Fig. 1b bottom). Thin agarose-gel coating effectively induced the lipid hydration in a saline solution, and also allowed the liposome formation with the in-vitro GFP synthesis solution.