Using surface resonance (SPR) as a sensitivity enhancer, this work describes the development of a transmissive multimode optical fiber sensor with a gold (Au) thin film that measures glucose concentration. The fiber's cladding was initially removed, and an Au layer was then sputtered onto its surface to simultaneously excite SPR and reflect light, making the SPR sensor extremely sensitive to changes in the environment's refractive index. A range of glucose concentrations, from 0.0001 to 0.5000 g/ml, were tested on the sensor. A maximum sensitivity of 161.302 nm/(g/mL) was attained for the lowest glucose concentration, while the highest concentration yielded a sensitivity of 312.000 nm/(g/mL). The proposed sensor's compact size, high sensitivity, good stability and practicality make it a promising candidate for a range of applications, including detecting diabetes.