Conformational changes of an oriented film of photosensitive proteins observed by polarized ATR infrared spectroscopy
- Resource Type
- Conference
- Authors
- Temperini, Maria Eleonora; Polito, Raffaella; Intze, Antonia; Baldassarre, Leonetta; Giliberti, Valeria; Ortolani, Michele
- Source
- 2021 46th International Conference on Infrared, Millimeter and Terahertz Waves (IRMMW-THz) Infrared, Millimeter and Terahertz Waves (IRMMW-THz),2022 47th International Conference on. :1-2 Aug, 2021
- Subject
- Aerospace
Bioengineering
Communication, Networking and Broadcast Technologies
Components, Circuits, Devices and Systems
Engineered Materials, Dielectrics and Plasmas
Fields, Waves and Electromagnetics
Photonics and Electrooptics
Signal Processing and Analysis
Proteins
Sensitivity
Surface waves
Films
Prototypes
Crystals
Silicon
- Language
- ISSN
- 2162-2035
We apply polarized Attenuated Total Reflection Fourier Transform Infrared spectroscopy (ATR-FTIR) to study conformational changes of the prototype photosensitive protein bacteriorhodopsin (BR). The experiments are performed on hundreds-nanometer-thick films of stacked membrane patches embedding BR molecules. Moreover, we report our results on functionalization of silicon surfaces that are preliminary to further experiments involving faced-down silicon substrates on ATR crystal in order to increase the sensitivity down to very thin protein films.