Summarizing Probe Intensities of Affymetrix GeneChip 3' Expression Arrays Taking into Account Day-to-Day Variability
- Resource Type
- Periodical
- Authors
- Magni, Paolo; Simeone, Angela; Healy, Sandra; Isacchi, Antonella; Bosotti, Roberta
- Source
- IEEE/ACM Transactions on Computational Biology and Bioinformatics IEEE/ACM Trans. Comput. Biol. and Bioinf. Computational Biology and Bioinformatics, IEEE/ACM Transactions on. 8(5):1425-1430 Jan, 2011
- Subject
- Bioengineering
Computing and Processing
Probes
Gene expression
Arrays
Correlation
Algorithm design and analysis
Bioinformatics
statistical analysis
Affymetrix microarray
experimental variability.
- Language
- ISSN
- 1545-5963
1557-9964
2374-0043
Microarray experiments are affected by several sources of variability. The paper demonstrates the major role of the day-to-day variability, it underlines the importance of a randomized block design when processing replicates over several days to avoid systematic biases and it proposes a simple algorithm that minimizes the day dependence.