Analysis of Fibrous Assembly Orientations from XFEL Diffraction Data
- Resource Type
- Conference
- Authors
- Wojtas, David H.; Seuring, Carolin; Ayyer, Kartik; Arnal, Romain D.; Meents, Alke; Mossou, Estelle; Pena, Gisel; Xavier, P. Lourdu; Barthelmess, Miriam; Forsyth, V. Trevor; Barty, Anton; Chapman, Henry N.; Millane, Rick P.
- Source
- 2018 International Conference on Image and Vision Computing New Zealand (IVCNZ) Image and Vision Computing New Zealand (IVCNZ), 2018 International Conference on. :1-6 Nov, 2018
- Subject
- Computing and Processing
Photonics and Electrooptics
Robotics and Control Systems
Signal Processing and Analysis
Diffraction
X-ray diffraction
Liquids
Graphene
X-ray imaging
Imaging
Scattering
XFEL
crystallography
fibrous assembly
imaging
biological system
- Language
- ISSN
- 2151-2205
The application of a new generation of x-ray sources called X-ray Free Electron Lasers (XFELs) to diffractive imaging has allowed structural studies of specimens not previously accessible. Specimens of reduced crystallinity are of particular interest, including fibrous nano-crystals and single fibrous molecules. Diffractive imaging experiments using XFELs generate large datasets of diffraction frames from specimens with random, unknown orientations. The orientation of each diffraction frame needs to be determined from features in the pattern in order to register and merge the dataset for subsequent structural analysis. Certain sample delivery techniques simplify this process by limiting the range of orientations a specimen may take. In this paper we consider two sample delivery techniques: a liquid jet and a fixed target on a silicon wafer. Orientations determined from diffraction patterns from each delivery method are classified in order to investigate the type of orientation present. This information also helps to characterize the quality of sample preparations and provides feedback valuable for designing future experiments.