Encapsulation of Micrornas in Exosomes for Efficient Intracellular Delivery by a Nanofluidic Platform
- Resource Type
- Conference
- Authors
- Yu, Zitong; Hao, Rui; Chen, Sihui; Zhang, Huitao; Hu, Shi; Ren, Jingyi; Hong, Yanhang; Liu, Bingrun; Zuo, Qisang; Yang, Hui
- Source
- 2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers) Solid-State Sensors, Actuators and Microsystems (Transducers), 2023 22nd International Conference on. :2137-2140 Jun, 2023
- Subject
- Bioengineering
Components, Circuits, Devices and Systems
Fields, Waves and Electromagnetics
Photonics and Electrooptics
Robotics and Control Systems
Encapsulation
Proteins
Micromechanical devices
Transducers
RNA
Loading
Medical treatment
Exosome nanoengineering platform
Exosomes
miRNAs encapsulation
Intracellular delivery
- Language
- ISSN
- 2167-0021
Here we report and validate a new strategy to package RNAs into exosomes using micro- and nanofluidic technology. We propose a nanofluidic constriction-based platform for miRNAs encapsulation into exosomes, referred to as the exosome nanoengineering platform (ExoNP). The device enables over 10 times microRNA-494 (miR494) enrichment in exosomes. When delivered intracellularly via these processed exosomes, the miR494 are biologically active in recipient cells to regulate downstream gene LRG1, further causing the repression of the proteins. Our results provide a proof-of-principle demonstration that the ExoNP can provide a straightforward and robust exogenous loading technique for packaging and intracellular delivery of therapeutic RNA molecules.