This study integrated smart garment, data acquisition device (DAD), iPad and remote server to develop an ECG measurement system for long-term healthcare monitoring in daily life. This system allowed the subjects to measure in a comfortable state, and their movements are not restricted. The experimental results showed that good ECG signals can be acquired from sleeping, sitting, standing and walking. Therefore, the system can not only be applied to daily long-term monitoring, but also has the opportunity for clinical application.