Evaluation of CD3+ T Cell Percentage, Function and its Relationship with Serum Vitamin D Levels in Women with Recurrent Spontaneous Abortion and Recurrent Implantation Failure
- Resource Type
- article
- Authors
- Abdolah Mousavi Salehi; Mehri Ghafourian; Afshin Amari; Mahvash Zargar
- Source
- Iranian Journal of Immunology, Vol 19, Iss 4, Pp 369-377 (2022)
- Subject
- interferon-γ
recurrent implantation failure
recurrent spontaneous abortion
t-cells
vitamin d
Biology (General)
QH301-705.5
- Language
- English
- ISSN
- 1735-1383
1735-367X
Background: Women afflicted with recurrent spontaneous abortion (RSA) and repeated implantation failure (RIF) may have immune abnormalities. The role of vitamin D has been demonstrated in the function of the immune system. Objective: To assess the percentage and function of CD3+ T cells and their relationship with the level of the serum vitamin D or 1,25-dihydroxy vitamin D3 (the active form of the vitamin) in women with RSA and RIF. Methods: In this case-control study, peripheral blood was obtained from the patient and the healthy control groups. The ratio of CD3+T cell and activated CD3+ CD69+T cell was investigated using flow cytometry. The serum levels of Interferon-γ (IFN-γ) and vitamin D were measured by ELISA. Results: The mean proportion of CD3+T cells in women with RSA increased significantly compared with the healthy control group (p