Determination of azelaic acid and potassium azeloycinate diglycinate in cosmetics by HPLC
- Resource Type
- article
- Authors
- XU Yong; HAN Jing; YU Ling; HUI Taicheng; HUANG Jun; PENG Xingsheng; ZHENG Rong
- Source
- Shanghai yufang yixue, Vol 34, Iss 12, Pp 1250-1256 (2022)
- Subject
- azelaic acid
potassium azeloycinate diglycinate
hplc
cosmetics
Medicine
- Language
- Chinese
- ISSN
- 1004-9231
ObjectiveAn HPLC method was established for the determination of azelaic acid and potassium azeloycinate diglycinate in cosmetics.MethodsThe samples were extracted with 60 mmol·L-1 sodium hydroxide water solution-methyl alcohol. After centrifugation and filtration, the analysis of azelaic acid and potassium azeloycinate diglycinate was performed with a SVEA C8(250 mm×4.6 mm, 5 μm) column, using 15 mmol‧L-1 potassium dihydrogen phosphate solution (pH=3.0) and acetonitrile for gradient elution at a flow rate of 1.0 mL·min-1. The analytes were detected with UV detector, and quantified by external standard curve.ResultsThe results showed a good linearity in the range of 5‒1 000 μg‧mL-1 with correlation coefficients (r) larger than 0.999. The detection limit of azelaic acid and potassium azeloycinate diglycinate (LOD) was 0.020% and 0.015%, respectively. The spiked recoveries were 87.66% to 108.96% with the relative standard deviation (RSD) of 0.6% to 3.3%.ConclusionThe method is simple, rapid and highly sensitive. It is suitable for the determination of azelaic acid and potassium azeloycinate diglycinate in cosmetics.