Introduction: Empathy is a quality that allows dentists to build an intersubjective relationship with their patients, which, among other benefits, contributes to the effectiveness of the treatment. Objective: To determine whether there is variability in empathy levels between two populations of dental students and to describe theoretically the general implications of this variability for intervention strategies. Materials and methods: Exploratory cross-sectional study. The study population consisted of 1st-5th year dental students from the Universidad Santiago de Cali, Colombia (n=610; N=647) and the Universidad San Sebastián, Chile (n=535; N=800). In both groups, empathy was measured using the Jefferson Scale of Empathy (S-Version) Scale. Descriptive statistics (mean and standard deviation) were used for data analysis. Internal consistency of data was estimated using Cronbach's alpha and the intraclass correlation coefficient. A factorial analysis of variance was performed, and three factors were studied: University (U), Course (C), and Sex (S). The statistical significance level used was α