LncRNA HOTTIP as a diagnostic biomarker for acute respiratory distress syndrome in patients with sepsis and to predict the short-term clinical outcome: a case-control study
- Resource Type
- article
- Authors
- Weitao Shi; Wang Zhu; Jiani Yu; Yingjun Shi; Yuliang Zhao
- Source
- BMC Anesthesiology, Vol 24, Iss 1, Pp 1-13 (2024)
- Subject
- Sepsis
HOTTIP
ARDS
Diagnostic
Predicts
Anesthesiology
RD78.3-87.3
- Language
- English
- ISSN
- 1471-2253
Abstract Background The present research aims to investigate the clinical diagnostic value of LncRNA HOXA distal transcript antisense RNA (HOTTIP) in acute respiratory distress syndrome (ARDS) of sepsis and its predictive significance for mortality. Methods One hundred eighteenth patients with sepsis and 96 healthy individuals were enrolled. RT-qPCR to examine HOTTIP levels. The incidence of ARDS and death was recorded. The diagnostic significance of HOTTIP in sepsis ARDS was examined using ROC and logistic regression analysis. The correlation between HOTTIP and disease severity was evaluated using Pearson’s coefficients. Kaplan-Meier analysis and COX regression were employed to examine the predictive significance of mortality. Validation of HOTTIP target miRNA by dual-luciferase assay. Results HOTTIP was persistently up-regulated in patients with ARDS sepsis than in patients without ARDS patients (P