Protocol to quantify enzymatic effects on vitreous liquefaction in porcine eyes using a transwell-plate system
- Resource Type
- article
- Authors
- Julian Wolf; Luís E. Sabage; Young Joo Sun; Vinit B. Mahajan
- Source
- STAR Protocols, Vol 3, Iss 4, Pp 101754- (2022)
- Subject
- Health sciences
Molecular biology
Protein biochemistry
Science (General)
Q1-390
- Language
- English
- ISSN
- 2666-1667
Summary: This protocol describes an ex vivo model to quantify enzymatic effects on vitreous liquefaction using porcine eyes in a transwell-plate system via induced syneresis. It provides a standardized dissection process and performs critical steps for gel-liquid separation with high precision, minimal tissue loss, and scalability. The protocol can be applied to other studies investigating vitreous liquefaction or gelatinous tissue analysis and can also serve to study vitreous liquefaction in vivo as it may occur during aging or disease progression. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.