Numerous phosphate occurrences are located in the Bohemian Cretaceous Basin (BCB) of the Czech Republic, within the Cenomanian–Turonian sequences. Small phosphate occurrences have been reported in the Upper Cenomanian, Lower Turonian, and Upper Turonian marine glauconitic siliciclasts. The phosphates are generally 2O5, CaO, F, Na2O, SO3, and CO2. Minor amounts of SiO2, Al2O3, K2O, and MgO are found, related to quartz and alumino-silicate impurities. Evidence of fossil microbial structures is revealed. The indices derived from rare earth elements (REE) indicate phosphogenesis at various redox conditions, ranging from anoxic to oxic, whereas the carbon stable isotopes of the apatite suggest generally reducing conditions. The critical and other valuable elements found in these Mid-Cretaceous phosphates include P2O5 (18.9–26.76 wt. %), F (1.67–3.25 wt. %), REE (325–1338 ppm), Y (74–368 ppm), and U (10.4–37.9 ppm). The investigation of the Turonian phosphate occurrences show that those located at the base of the Bílá Hora Formation (earliest Turonian) are the most persistent in the southern margins of the BCB, and found in localities extending for about 200 km. They were developed at the onset of the Early Turonian global transgression and are strata-bound to the base of the Bílá Hora Formation. Future exploration for marine sedimentary phosphorites should focus on thicker and better developed deposits at the base of the Turonian sediments as the main target.