Intracranial pressure and optic disc changes in a rat model of obstructive hydrocephalus
- Resource Type
- article
- Authors
- Snorre Malm Hagen; Sajedeh Eftekhari; Steffen Hamann; Marianne Juhler; Rigmor H. Jensen
- Source
- BMC Neuroscience, Vol 23, Iss 1, Pp 1-9 (2022)
- Subject
- Hydrocephalus
Intracranial pressure
Papilledema
Epidural
Kaolin
Fluid-filled system.
Neurosciences. Biological psychiatry. Neuropsychiatry
RC321-571
Neurophysiology and neuropsychology
QP351-495
- Language
- English
- ISSN
- 1471-2202
Abstract Background The kaolin induced obstructive hydrocephalus (OHC) model is well known for its ability to increase intracranial pressure (ICP) in experimental animals. Papilledema (PE) which is a predominant hallmark of elevated ICP in the clinic has not yet been studied in this model using high-resolution digital fundus microscopy. Further, the long-term effect on ICP and optic nerve head changes have not been fully demonstrated. In this study we aimed to monitor epidural ICP after induction of OHC and to examine changes in the optic disc. In addition, we validated epidural ICP to intraventricular ICP in this disease model. Method Thirteen male Sprague-Dawley rats received an injection into the cisterna magna containing either kaolin-Ringer’s lactate suspension (n = 8) or an equal amount of Ringer’s lactate solution (n = 5). Epidural ICP was recorded post-operatively, and then continuously overnight and followed up after 1 week. The final epidural ICP value after 1 week was confirmed with simultaneous ventricular ICP measurement. Optic disc photos (ODP) were obtained preoperatively at baseline and after one week and were assessed for papilledema. Results All animals injected with kaolin developed OHC and had significant higher epidural ICP (15.49 ± 2.47 mmHg) compared to control animals (5.81 ± 1.33 mmHg) on day 1 (p